Forum

«Challenges in Flow-Biocatalysis»

Enzyme immobilisation offers an opportunity to render biocatalysts heterogeneous and allows their recovery from abatch reaction. At the same time, enzyme immobilisation allows for packing such biocatalyst in a reactor and runbiotransformation in continuous.

Enzyme immobilisation offers an opportunity to render biocatalysts heterogeneous and allows their recovery from abatch reaction. At the same time, enzyme immobilisation allows for packing such biocatalyst in a reactor and runbiotransformationin continuous. While this sounds straight forward, there is no single immobilisation method thatsuits every enzyme and tailoring is essential to deliver highly stable and active biocatalysts. In addition, severalbiocatalysts require exogenous cofactors andwhen this is transferred in flow, even if the cofactor can be recycled, itwill eventually be lost downstream. Cofactor co-immobilisation offers an opportunity to overcome this hurdle. But flowbiocatalysis, in packed-bed reactors, faces a number of challenges,such as possible interaction of thesubstrate/product with the enzyme carrier, insolubility of the substrate/product in aqueous medium, as well as enzymethat are oxygen dependent and struggle in terms of efficiency in a sealed environment. In this talk, aseries ofchallenges and solutions will be presented, as well as yet unsolved issues.

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Speakers (1)

Francesca Paradisi, University of Bern

Francesca Paradisi, University of Bern

Professor of Sustainable Pharmaceutical Chemistry