Maximizing Fluorescent Signal Using Variable Bandwidth
Maximizing Fluorescent Signal Using Variable Bandwidth Monochromators for Plate Reader-Based Assay Optimization: Presented in the Webinar by BioTek, now a part of Agilent.
One of the challenges with multiplexing fluorophores in biological assays is being able to reliably distinguish the signal of one fluorophore from another. The complication of working with fluorophores with significant spectral overlap is exacerbated when using fluorescent filters or fixed bandwidth monochromators, which provide little flexibility in optimizing signal and minimizing background. Variable bandwidth monochromators provide a powerful alternative that enables fine control over the light range by which fluorophores are excited and emitted light is collected, which in turn allows signal to be more reliably deciphered. BioTek offers a fleet of hybrid-plate readers including the Synergy H1, Neo2, and Cytation, which offer the most flexibility when assaying with multiple fluorescent probes.
You can watch the webinar, available on demand, here.