Story CelVivo – 3D Cell Culture Technology The CelVivo system enables cells to take advantage of an environment in which they develop a functionality which is much more like that of an intact living organism than that offered by two-dimensional cell culture. This trend towards 3D cell culture can no longer be overlooked. Over the past decade, the number of publications in the field of 3D culture has grown exponentially. While around five thousand publications on this topic existed a decade ago, today there are more than 22,000 publications addressing three-dimensional cell culture. And new publications are currently running at almost three thousand a year.The reason for this trend is the substantial limitations which apply to two-dimensional cell culture. Cells grown under flat (2D) conditions do not behave like the cells which grow in the natural organism. The activity, growth and intercellular communication observable in the host are all different from those same attributes as displayed via 2D cell culture. In addition, the trypsinization of cells during passage destroys their extracellular matrix and thus part of the mechanism controlling cell activity. As a result, only limited physiologically relevant data can be obtained from a 2D cell culture.Better results with the microgravity system The ClinoStar is a CO2 incubator with a microgravity system which makes the cultivation of three-dimensional structures easy and reproducible. And this gain in the in-vivo-like physiological properties greatly improves the informative value of scientific studies. A Klinostat can thus increase the effectiveness of toxicological studies – both acute and chronic (single dose or repeated doses). It can also be used in cancer research, regenerative medicine and for the discovery of new drugs.The ClinoStar enables the production of uniform, reproducible and functional spheroids and organoids. These spheroids realistically imitate the function, structure and architecture of the living organism. Under laboratory conditions, this creates an unprecedented correlation between cell culture and cell function.